Isolated metaphase chromosomes. II. Proteins of Chinese hamster chromosomes.
نویسندگان
چکیده
منابع مشابه
Proteins of metaphase chromosomes and interphase chromatin.
Metaphase chromosomal and interphase chromatin proteins from cells of two species have been compared by polyacrylamide gel electrophoresis. Consistent, common changes in the quantitative distribution of the nonhistone chromosomal proteins are observed in both species. Proteins of ca. 65,000 and 68,000 MW are enriched in interphase chromatin while proteins of ca. 50,000 and 200,000 are more prom...
متن کاملNucleosomes in metaphase chromosomes.
Previous studies of the structure of metaphase chromosomes have relied heavily on electron micrography and have revealed the existence of a 10-nm unit fiber that is thought to generate the native 23-30-nm fiber by higher order folding. The structural relationship of these metaphase fibers to the interphase fiber remains obscure. Recent studies on the digestion of interphase chromatin have revea...
متن کاملAsynchronous Duplication of Chromosomes in Cultured Cells of Chinese Hamster
Chromosome duplication (DNA synthesis) was studied in cultured cells of Chinese hamsters by means of autoradiography following thymidine-H(3) incorporation. The technique used was to expose an asynchronously dividing population of rapidly growing cells for a 10 minute interval to a medium with thymidine-H(3). Cells were then transferred to a medium with excess unlabeled thymidine. The populatio...
متن کاملIsolated Chromosomes
The chromatin threads, which we described briefly several years ago (1), have since then been studied carefully both morphologically and chemically. We now consider these microscopic threads of chromatin to be isolated chromosomes (2). From certain animal cells such masses of isolated chromosomes can be prepared that, with respect to quantity of material available, no special methods are requir...
متن کاملIsolation of Metaphase Chromosomes from Hela Cells
The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes co...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1979
ISSN: 0022-1554,1551-5044
DOI: 10.1177/27.1.374609